The construction of 401 will lead to some significant deviations for drivers beginning on Sunday night.
The west ramp to Highway 8 and King Street East will close at 10 o'clock.
This means that drivers will only be able to go to Hevers Road or Homer Watson.
The left and center lanes of the two lanes to the east and west will also be closed on Monday between Hespeler Road and Highway 8.
These reductions will begin at 8 am. and continue until Thursday at 6 o'clock in the morning.
Drivers are advised to plan an alternative route.