Baltimore, MD, May 22, 2019 – Stem cell-based therapies to strengthen heart muscle and treat other diseases begin to show promise in clinical trials in humans. However, in addition to monitoring the clinical outcomes, the lack of a repeatable, time sensitive and non-invasive tool for assessing the effectiveness of transplanted cells in the target organ slowed progression in the stem cell domain.
Researchers at the University of Maryland Medical School (UMSOM), University of Pennsylvania and Emory University say a blood test can be used to track the efficiency of transplanted stem cells. They aim to achieve their goal by analyzing small cellular components called exosome secreted by the transplanted stem cells in the recipient's blood. They tested their theory in models of heart attack or myocardial infarction after transplantation of two types of human cardiac stem cells and observation of their circulating exosomes. Researchers have found that circulating exosomes deliver cellular components to the heart muscle cells, resulting in heart recovery. The results are published in the magazine Science Translational medicine,
"Exosomes contain the signals from the cells from which they originate – proteins, as well as nucleic acids and micronucleic acids (miRNAs) – that affect receptor cells and remodel or regenerate the organ to which we target," says study co-author Sunjay Kaushal Professor of Surgery at UMSOM and Director of Pediatric Cardiac Surgery at the Children's Hospital of the University of Maryland. "We now have a tool to determine if stem cell therapy will be effective for an individual patient, not just for the heart, but for every organ that has received stem cell therapy."
By the blood test, which researchers call a "liquid biopsy," researchers are investigating human cardiac spheres (CDCs) and cardiac progenitor cells (CPCs) transplanted into the heart of rats after myocardial infarction. Blood plasma exosome concentrations are compared seven days after transplantation.
After purification of exosomes obtained from CDC / CPC, the researchers found that exosomes contained miRNAs associated with heart muscle repair. In addition, they found that the COPD and CDCs produced in the culture differed in content from exosomes produced from transplanted cells in the living organism.
"Our study should be considered the first step in understanding what stem cells do, but an important point is that the cells we identified as reacting change their gene expression, behaviors and secretions," said Dr. Sushi Sharma, UMSOM Surgery assistant. "By using these biomarkers we can understand the mechanism and the extent of recovery."
P. Saha, S. Sharma, L. Korutla, S. Dathla, F. Shaia-Taheri, R. Mishra, G. Beigham, M. Sarkar, D. Morales, G. Beat, M. Gunacekaran, K. Ambestha, MJ Arfat, D. Li, A. Habertuer, R. Hu, M. O. Platt, P. Yang, M. E. Davis, P. Valabhaioshula, S. Kauşal. "Circulating exosomes derived from transplanted progenitor cells support the functional recovery of the ischemic myocardium." Sci. Transl. Med. 11, eaau1168 (2019)
About the Maryland Medical University
Now in its third century, Maryland University Medical School was hired in 1807 as the first state medical school in the United States. Today it continues to be one of the world's fastest growing, top-ranking biomedical research businesses – with 43 academic departments, centers, institutes and programs; and lecturers from over 3,000 doctors, health scientists and associates, including members of the National Medical Academy and the National Academy of Sciences, and an excellent winner of the Albert Lasker Prize in Medical Research. With an operating budget of over $ 1 billion, the Medical Faculty works in close partnership with the Medical and Medical System of the University of Maryland to provide scientific-intensive, academic and clinical-based care for more than 1.2 million patients each year. The Faculty of Medicine, ranked 8th among public medical schools in the field of research and development, is an innovator in translation medicine with 600 active patents and 24 start-ups. The school operates on a local, national and global level, with research and treatment facilities in 36 countries around the world. Visit http: // www.
About the Medical Center of the University of Maryland
Maryland University Medical Center (UMMC) consists of two Baltimore hospitals: a 800-bed hospital, the UMMS 14-hospital medical institution, and a 200-bed community hospital, the Campus of the United States UMMC Midtown. UMMC is a national and regional referral center for traumas, cancer care, neuroscience, cardiac care, diabetes and endocrinology, women's and children's health and one of the largest organ transplant programs in the country. All doctors in the leading hospital staff are physicians at the Maryland University School of Medicine. At the UMMC Midtown Campus, physicians at the faculty work with community doctors to provide patients with the highest quality care. The UMMC Midtown Campus was founded in 1881 and is located a mile from the university campus hospital. For more information, visit http: // www.
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